The influence of ischemia limb reperfusion on mineralization condition under connected abdomino-skeletal injury and massive treatment

Abstract

The influence of limb reperfusion in the model of concomitant injury and massive blood loss on the processes of mineralization was studied. The experiment was conducted with 130 adult male Wistar rats weighing 190–220 g, which were on a standard diet of vivarium. All animals were divided into four groups i.e. control and three experimental ones; the control group contained intact animals (10 animals); the first experimental group consisted of animals, which modeled a hip fracture, massive external bleeding and ischemia-reperfusion of the lower extremities (40 animals); the second group were made up of animals that were modeled for skeletal trauma, massive external blood loss, and closed trauma to the abdominal organs (40 animals); the third group consisted of animals that modeled closed abdominal trauma, skeletal trauma, massive external blood loss, and lower extremity ischemia-reperfusion (40 animals). The animals of the experimental groups were removed from the experiment under conditions of thiopental sodium anesthesia by total blood flow from the heart 3, 7, 14 and 21 days after trauma modeling. The bone of the lower extremities was removed for the study. In the bone homogenate, the level of alkaline phosphatase, acid phosphatase was determined and a mineralization index was calculated based on these data. It has been established that limb reperfusion in the model of combined abdominal-skeletal trauma has a potentiating effect on changes of alkaline and acid phosphatase indices, which is manifested by a significant increase in their activity during the 21 days after the traumatic period. Changes in the index of mineralization index among experimental animals indicate a negative impact of ischemia reperfusion on reparative processes in bone tissue, which outlines directions for further research.