Abstract

A dual effort investigation was conducted to study (a) the naturally occurring variation in same-donor human hand odor samples over time and (b) the accuracy in associating same-donor human hand odor samples. Hand odor samples were collected from 8 donors throughout 5 sampling sessions; samples were collected in triplicate and analyzed by HS-SPME-GC-MS at each sampling session. The resulting human hand odor profiles were analyzed to investigate (a) the variability of human hand odor profiles as a function of time and (b) the ability to determine the source origin of human hand odor samples, determining samples to be from the same source or different sources. The researchers observed greater variation in 2-dimensional human scent profile patterning schemes among inter-day, inter-subject samples and less variation in inter-day, intra-subject samples. Although intra-subject samples revealed less variation than inter-subject samples, there was still notable variability among inter-day, intra-subject human scent profiles, with an observed time dependency. Two proof of concept models for the source determination of human hand odor samples were developed with maximum performance measuring TPR = 0.817/ FPR = 0.308 and TPR = 1.000/ FPR = 0.206 for models one and two, respectively. The study quantified same-donor human hand odor profile variation over time displayed within a larger goal of determining sample source origin.

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