Abstract
Objective In this study, we explored the influence of single nucleotide polymorphism (SNP) in the noncoding region of intercellular adhesion molecule 1 (ICAM1) gene on the occurrence and metastasis of primary hepatocellular carcinoma (PHC). Methods Sanger sequencing was used to analyze the genotypes of rs3093032, rs923366, and rs281437 locus in the 3′untranslated region (UTR) of the ICAM1 gene. The level of plasma ICAM1 was analyzed by enzyme-linked immunosorbent assay (ELISA). Results After adjusting for risk factors such as BMI, smoking, drinking, family history of tumors, and hepatitis B virus test results, the CT genotype at rs3093032 of the ICAM1 gene (OR = 0.19, 95% CI: 0.08-0.44, P < 0.01), dominance model (OR = 0.23, 95% CI: 0.11-0.48, P < 0.01), and T allele (OR = 0.27, 95% CI: 0.14-0.53, P < 0.01) were related to the reduced risk of PHC susceptibility. rs923366 locus CT genotype (OR = 0.63, 95% CI: 0.44-0.90, P = 0.01), TT genotype (OR = 0.23, 95% CI: 0.10-0.53, P < 0.01), dominant model (OR = 0.55, 95% CI: 0.39-0.77, P < 0.01), recessive model (OR = 0.28, 95% CI: 0.12-0.62, P < 0.01), and T allele (OR = 0.55, 95% CI: 0.42-0.73, P < 0.01) were related to a reduction in the risk of PHC susceptibility. rs281437 locus CT genotype (OR = 2.08, 95% CI: 1.40-3.09, P < 0.01), TT genotype (OR = 5.20, 95% CI: 2.22-12.17, P < 0.01), dominant model (OR = 2.45, 95% CI: 1.69-3.54, P < 0.01), recessive model (OR = 4.32, 95% CI: 1.86-10.06, P < 0.01), and T allele (OR = 2.46, 95% CI: 1.79-3.38, P < 0.01) were significantly related to the increased risk of PHC susceptibility. SNPs at rs3093032, rs923366, and rs281437 of the ICAM1 gene were significantly correlated with TNM stage and tumor metastasis of PHC patients (P < 0.05). Conclusion SNPs at rs3093032, rs923366, and rs281437 in the 3′UTR region of the ICAM1 gene are related to the occurrence and metastasis of PHC.
Highlights
Primary hepatocellular carcinoma (PHC) is one of the most common malignant tumors in the world, and its morbidity and mortality are among the top malignant tumors in the world [1, 2]
We explored the influence of single nucleotide polymorphism (SNP) in the noncoding region of intercellular adhesion molecule 1 (ICAM1) gene on the occurrence and metastasis of primary hepatocellular carcinoma (PHC)
The results of one-way ANOVA showed that body mass index (BMI), smoking, drinking, family history of tumors, and hepatitis B virus test results were related to PHC susceptibility, including BMI ≥ 25 kg/m2, smoking, Table 1: Comparison of clinical characteristics between PHC patients and controls
Summary
Primary hepatocellular carcinoma (PHC) is one of the most common malignant tumors in the world, and its morbidity and mortality are among the top malignant tumors in the world [1, 2]. The cause and pathogenesis of hepatocellular carcinoma have not yet been fully elucidated. With the progress of molecular biology, virology, and genetics, researchers have found that the occurrence of hepatocellular carcinoma is related to the interaction of environmental and genetic factors [6, 7]. Important risk factors for hepatocellular carcinoma include chronic hepatitis B virus and chronic hepatitis C virus infection, alcohol abuse, chromosomal instability, oxidative damage, DNA methylation genetic signal transduction pathway obstacles, and gene polymorphisms.
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