Abstract
The preservation of physiological transport of ions and water content is particularly important for maintaining the skin barrier, touch and pain stimuli, as well as the initiation of skin regeneration processes, especially after treatments associated with breaking skin continuity and wound healing difficulties. The aim of the study was to assess changes in ion transport, measured as values of transepithelial electric resistance and potential difference in stationary conditions and during mechanical-chemical stimulations, depending on the hydration status of isolated rabbit skin specimens. The specimens were divided into five groups: control (n = 22), dehydrated in 10% NaCl (n = 30), rehydrated after dehydration (n = 26), dried at 37°C (n = 26), and rehydrated after drying (n = 25). Dehydrated tissue samples showed altered resistance compared to the control; this change was maintained regardless of rehydration. In the dehydrated samples, changes in the measured electric potential were also noted, which returned to values comparable with the control after rehydration. Dehydrated skin, regardless of the cause of dehydration, responds with changes in the transport of sodium and chloride ions and the altered cellular microenvironment. It could influence the perception of stimuli, particularly pain, and slow down the regeneration processes.
Highlights
The level of moisture and hydration of the skin is important for effective receptor stimulation, by pain stimuli, as well as collagen and elastin production, skin regeneration and maintaining a healthy appearance of the skin [1,2,3,4]
Tissue samples dehydrated with a 10% NaCl solution (Deh) had a median resistance of 352 O/cm2, and this value was lower in a statistically significant manner compared to Ctr and Dr The skin samples rehydrated after dehydration (RDeh) or rehydrated after drying (RDr) had lower resistance values in comparison with Ctr, Deh and Dr
We proposed two methods of dehydration of skin samples prepared in a way that conserved the layered tissue structure
Summary
The level of moisture and hydration of the skin is important for effective receptor stimulation, by pain stimuli, as well as collagen and elastin production, skin regeneration and maintaining a healthy appearance of the skin [1,2,3,4]. A properly hydrated skin is a barrier for microorganisms and external factors, while retaining the ability of physiological exfoliation, and has the capacity of receiving external stimuli [5,6,7,8,9,10]. Dehydrated skin becomes scratchy, mat, flushed, prone to exfoliation, and the occurring micro-damage leads to increased permeability and influx and activation of immunocompetent cells [4, 9, 11,12,13]. Decreased water content causes accumulation of corneocytes on the epidermal surface and disrupts exfoliation [3, 5, 14].
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