Abstract
BackgroundIt is well known that ABO blood group system incompatible kidney transplantation (ABOi-KT) is an effective strategy for end-stage renal disease. The main barrier for ABOi-KT is how to keep host B cell activation and blood group antibody titer in low levels. Moreover, the mechanism of B cell activation induced by blood group antigen was unclear in ABOi-KT.ResultsIn this study, HK2 cells were identified to express blood group B antigen when cocultured with lymphocytes of blood group A. Optical microscope observation demonstrated that HK2 cells in coculture group gradually decreased. Furthermore, flow cytometer assay identified that T cell phenotypes (CD3+, CD3+CD4+ and CD3+CD8+) had no significant change and B cell phenotypes (CD19+ and CD138+) were all significantly enhanced (3.07 and 3.02 folds) at day 4. In addition, immunoturbidimetry analysis demonstrated that blood group B antibody was significantly increased to 2.35 fold at day 4, IgG was significantly increased to 3.60 and 2.81 folds at days 4 and 8 respectively, while IgM had no significant change at the measured time points.ConclusionsTaken together, B cells were activated and secreted blood group B antibody after treatment with HK2 expressing blood group B antigen. The results of this study maybe useful for further determination of the mechanism of B cell activation after ABO incompatible kidney endothelial cells stimulation.
Highlights
It is well known that ABO blood group system incompatible kidney transplantation (ABOi-KT) is an effective strategy for end-stage renal disease
Identification of blood group antigen type of HK2 cells To research the change of B cell activation by blood group antigens of kidney endotheliocytes stimulated in vitro, the blood group antigen type of the HK2 cell line was analyzed by immunohistochemistry (Fig. 1)
As for results, none of the imaged HK2 cells were stained after incubation with Phosphate buffer solution (PBS) and mouse monoclonal to anti
Summary
It is well known that ABO blood group system incompatible kidney transplantation (ABOi-KT) is an effective strategy for end-stage renal disease. The main barrier for ABOi-KT is how to keep host B cell activation and blood group antibody titer in low levels. The mechanism of B cell activation induced by blood group antigen was unclear in ABOi-KT. ABOi-KT is an effective replacement therapy for endstage kidney disease [1,2,3], in which the key for graft survival is to eliminate the host blood group antibodies prestored in peripheral blood of recipients [4, 5]. The allograft in part of ABOi-KT recipients survived without rejection when the blood group antibody titer was gradually increased to the preoperative level [6]. The mechanism of B cells activation in ABOi-KT was unclear
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