Abstract
Objective: To research the expression of hypoxia inducible factor-1 alpha (HIF-1 alpha) on the apoptosis and number of T lymphocyte in Peyer’s patches after severe burn on plateau in rats. Methods: Wistar rats (n = 130) were subjected to deep thickness burn injury (30% TBSA, III degree), at two different altitudes. 60 of them were given delayed fluid resuscitation (DFR, n = 30 at each altitude) 6 h after burn at different altitude; 60 of them were carried out immediate fluid resuscitation (IFR, n = 30 at each altitude); 10 rats were subjected to 37°C warm water as sham burn (SG, n = 10). The Peyer’s patches were harvested from the ileum of rats at different time point after burn respectively. The expression of HIF-1 alpha, CD3(+) and the apoptosis and number of T lymphocyte in Peyer’s patches were detected by tissue microarray technology and immunohistochemistry. Results: The apoptosis was higher in DFR group than that in IFR group. The increase in HIF-1 alpha expression was observed mainly on cell nucleus in T lymphocytes. The expression levels of HIF-1 alpha in Peyer’s patches were much higher in DFR group and IFR group than those in SG, and they were higher at high altitude (3848 metres) than those at lower altitude (1517 metres), and also higher in DFR group compared with IFR group (all P + in Peyer’s patches were much lower in DFR group and IFR group than those in sham group, and the lowest value appeared at 12 hours after burn (all P
Highlights
Hypoxia is a well-known cause of cell injury at plain, with important pathological implications in many disease processes, including cerebral ischemia and myocardial infarction [1]
Hypoxia results in adaptationally appropriate alterations of gene expression through the activation of hypoxia-inducible factor-1 (HIF-1) to overcome any shortage of oxygen
HIF-1 is a basic helix-loop-helix/Per-ARNT-Sim protein consisting of Hypoxia-inducible factor-1α (HIF-1α) and HIF-1β subunits
Summary
Hypoxia is a well-known cause of cell injury at plain, with important pathological implications in many disease processes, including cerebral ischemia and myocardial infarction [1]. Cells in hypoxia expressed a variety of adaptive or death gene products to satisfy altered metabolic demands or to remove irreversibly damaged cells [2]. It was reported that CD3positive human T cells accumulating in inflammatory tissue and expressing HIF-1α, indicating a role of hypoxia-mediated signals in regulation of T cell function. There was no report about the effects of HIF-1α expression on the apoptosis and proliferation of T lymphocytes in Peyer’s patches PB at plateau. A burn model with delayed resuscitation was duplicated at sub-plateau (1517 m above sea level) and plateau (3848 m above sea level) and the effects of HIF-1α expression on the lymphocytes in Peyer’s patch was observed
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