Abstract
We used complexes between a fourth generation polyamidoamine (PAMAM) dendrimer and one of two heterocyclic compounds — 1-(6-hydroxyhexyl)-3-(5-phenyl-isoxazole-3-yl)-urea or 5-phenyl-isoxazole-3-carboxylic acid — to reduce oxygen consumption in transverse slices of the hippocampus taken from 4-week old male rats. In vitro electrophysiological experiments revealed that the inhibitory effect of the hypoxic state on the evoked responses was enhanced in the presence of the complexes. The data were analyzed in terms of the potential antitumor effects of these complexes.
Highlights
Until recently, studies on hyperbaric oxygen treatment and cancer focused on whether increased oxygen levels could act as a cancer promoter [1]
Electrophysiological experiments After the preincubation period, the subsequent infusion of artificial cerebrospinal fluid (ACSF) saturated with carbogen revealed a gradual stabilization of evoked electrical responses in the stratum radiatum and stratum pyramidale
The results show that the hypoxic stimulus was accompanied by a reduction in the excitatory postsynaptic potentials (EPSP) amplitude and a short-term increase in the amplitude of population spikes (PS), followed by a catastrophic fall in EPSP to its minimum at 4 to 5 min (Figs 1B and 2C)
Summary
Studies on hyperbaric oxygen treatment and cancer focused on whether increased oxygen levels could act as a cancer promoter [1]. The aim of this study was to elucidate how the presence of dendrimer– heterocyclic compound complexes in brain tissue would affect the functioning of its signaling systems under hypoxic conditions. The continued presence of intercellular communication has been established both in brain slices and in cell culture [10, 11]. Hippocampal cells are involved in the control of memory and learning. They play their role by transmitting signals between the CA3 and CA1 regions. Intercellular communication begins to break down early, but it is difficult to establish the functional consequences of such changes in the initial stage of the pathological process. We evaluated the effects of heterocyclic compound–PAMAM dendrimer complexes on the intercellular communication in hippocampal slices in a state of hypoxia
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