The influence of gold nanoparticles on intercellular adhesion molecule-1 and transforming growth factor-β of colon carcinoma bearing mice before and after the electric knife treatment

Abstract

Objective To observe the influence of gold nanoparticles on intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-β (TGF-β) of colon carcinoma bearing mice before and after the electric knife treatment. Methods The experiment was divided into different concentrations of gold nanoparticles-treated groups and the saline control group. After the establishment of colon carcinoma bearing mice model, 0.2 ml solution in each group was injected via the tail vein every other day for 14 days. Then the transplanted tumors were taken out to measure the weight. The concentrations of serum ICAM-1 and TGF-β (3 days before and 3 weeks after the surgery) also were determined by enzyme linked immunosorbent assay (ELISA). Results Three days before surgery, the ICAM-1 concentration in 3 groups (control, 2.5 and 5.0 μg/ml gold nanoparticles) were (7.41±0.40), (5.32±0.35) and (3.84±0.42) ng/ml respectively (F=125.302, P=0.000). Three weeks after the surgery, the ICAM-1 concentration in 4 groups (sham operation group, simple operation group, 2.5 μg/ml gold nanoparticles+ operation group and 5.0 μg/ml AuNPs+ operation group) were (15.46±1.71), (3.44±0.37), (1.76±0.61) and (0.92±0.33) ng/ml respectively (F=313.424, P=0.000). Effects of gold nanoparticles on TGF-β concentration were similar to the above results. In addition, the xenograft weight in gold nanoparticles-treated groups was significantly different from that in the control group in a dose-dependent manner (F=106.125, P=0.000). Conclusion Gold nanoparticles can reduce serum ICAM-1 and TGF-β concentrations before and after the electric knife treatment, also reduce colon carcinoma mouse xenograft weight in vivo. Key words: Colon carcinoms; Gold nanoparticles; Intercellular adhesion molecule-1; Transforming growth factor-β