The influence of glycerol level for freezing medium on the quality of ram semen

Abstract

Research on the use of glycerol levels as a cryoprotectant in the semen freezing process of Palu ram has been carried out at the Reproduction Laboratory of the Faculty of Animal Husbandry and Fisheries, Tadulako University. The purpose of this study was to determine the best level of glycerol to maintain the quality of spermatozoa after thawing. This study used semen from 2.5 years old rams. The semen collection process was carried out two times a week. The treatments in this study were glycerol levels of 4%, 6% and 8% for P1, P2 and P3 respectively. The data was analyzed using analysis variance, the differences between treatments was analyzed by multiple range test Duncan. Based on the results of the study showed that the addition of glycerol at a level of 6% was able to maintain the best spermatozoa motility (45.42%) followed by a level of 9% (41.14 %) and a level of 3% (40.29). As a conclusion, that the level of glycerol significantly effected on motility, viability and abnormality.