The influence of Glutathione‐S‐transferase(GSTs) polymorphisms on the level of oxidative DNA damage, antioxidant status and lipid profiles in healthy Korean adults

Abstract

Human glutathione‐S‐transferases (GSTs) are known as phase □ enzymes involved in the detoxification to protect oxidative stress. This study compares the contribution of GSTT and GSTM genetic polymorphism on the susceptibility to oxidative damage or to antioxidant status on healthy subjects depending on their smoking status. The degree of DNA damage in smokers (n=48) were not significantly different than that of non‐smokers(n=197). Similarly, the levels of total radical trapping antioxidative potential(TRAP), erythrocyte SOD, GST‐Px and catalase showed slight increase in the GSTT1, GSTM1 null type, but, was not significantly different. Plasma HDL‐Chol was significantly higher in the GSTM1 null type(p<0.05), while the other parameters were not different. In non‐smokers, GSTT1 null genotype had significantly higher DNA damage than the wild type(p<0.05). Plasma TRAP and the levels of antioxidant enzymes did not show any significant difference depending on the genotype. When all these effect was adjusted with smoking status, GSTM1 wildtypes and GSTT1 null types showed lower overall oxidative stress. (This study was supported grant, 2009‐0069893, from the National Research Foundation of Korea)