THE INFLUENCE OF ENVIRONMENTAL LIGHTING ON THE GROWTH AND PROMETAMORPHIC DEVELOPMENT OF LARVAL RANA PIPIENS.

Abstract

This study was designed to investigate whether the larval development of an anuran amphibian could be modified by raising the animals in continuous light or darkness instead of under conditions of diurnal illumination, and to quantify the effects of these treatments at various intervals during this period of development. Larvae of the frog, Rana pipiens, were raised through metamorphosis under conditions of constant light, constant darkness, or diurnal lighting. As measured by stages of development, body weight, tail length and body length at 20-day intervals, no significant differences in growth rate or metamorphic change were observed until near the middle of the prometamorphic period, which began at approximately the 50th day of development. After midmetamorphosis, a significant acceleration in the measured parameters was seen for the animals raised in conditions of constant light in comparison with those in constant darkness. Those with diurnal lighting were intermediate. These results suggested that light, or its absence, can respectively stimulate or retard amphibian metamorphosis in late larval stages after the hypothalamo-hypophyseal-thyroid axis has matured. Neither continuous light nor continuous darkness during larval development prevented the transformation from tadpole to frog.