Abstract
Abstract 1. 1. Fatty acid synthetase from liver of cold and warm adapted flounder and rabbit was purified to homogenity and compared. 2. 2. The mol. wt of the cold and warm flounder enzyme was estimated to be about 457,000. 3. 3. The kinetic properties were found to be similar for warm and cold adapted flounder liver enzyme and not different from the rabbit liver enzyme when measured at 5, 10, 15, 20 and 37°C. 4. 4. Palmitic acid was the main product of both the flounder and rabbit enzyme, but significant amounts of butyric acid were also synthesized. The product composition did not change for any of the enzymes tested when the incubation temperature was changed. 5. 5. It was concluded that fatty acid synthetase from flounder liver is similar to mammalian fatty acid synthetase with regard to molecular weight and kinetic properties.
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