Abstract
The influences of electromagnetic field with low frequency irradiating by high-voltage transmission lines on signal transduction of spleen cells in the rates have been studied by molecular-biology techniques. The spleen cells are extracted from skilled rates, which are exposed in the electromagnetic field of high-voltage transmission lines with 4000 V/m and 0.09-0.1 G about 400 days. The quantities or levels of phosphorylation of signal transducer and activator of transcription (STAT3) in JAK-STAT signal transduction pathway of spleen cells, are detected and compared by the immunoblotting and immunobiochemistry, when the cells are stimulated and unstimulated by IL-2, respectively. The results show that the expression quantities of phospho-STAT3 in spleen cell stimulated by IL-2 differ from that in the unstimulated cell. The former is significantly large than the latter for the experimental group, but the controlled group is not.This shows that signal transduction of cell is affected by this electromagnetic field. The spectra of infrared absorption for the general G-proteins participating this signal transduction of cell for the experimental and controlled groups, which are measured by Nicoletic FT-IR 670 spectrometer, are obviously different both the intensity and frequency of these peaks. This shows that molecular structure or conformation of the proteins changes under influence of the electromagnetic field of high-voltage transmission lines, which results just to above changes of JAK-STAT signal transduction of cell.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
