Abstract
Many of the DNA and RNA puffing changes observed in Rhynchosciara during the prepupal period have been induced in younger larvae by injection of ecdysterone. However, the dose of hormone necessary for this induction is high, especially in the large cells of the proximal region of the gland. There are differences in the amplification and puffing response from that observed during normal development. Particular similarities and differences with possible explanations for the differences are discussed. Preceding and during the amplification which occurs at certain chromosomal regions, ecdysterone induces DNA synthesis along the entire chromosome. This induction of general DNA synthesis can occur independently of the amplification process. It appears to be similar in pattern to that occurring normally toward the end of larval life. — The normal prepupal behavior of Rhynchosciara was not induced by injection of ecdysterone into larvae of any age thus far examined.
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