The influence of discoidin domain receptor 2 on the persistence length of collagen type I fibers

Abstract

Collagen fibers in the vertebrate tissue are responsible for its tensile strength. A disruption in the morphological or mechanical properties of collagen fibers is bound to impact tensile strength and contractility of tissues and affect several cellular processes. We had recently established that binding of discoidin domain receptor (DDR2) with collagen type I results in disruption of the native structure and morphology of collagen fibers. In this study we investigate if DDR2 affects the mechanical properties of collagen fibers. We used an analytical approach to determine the persistence length (PL) of collagen fibers from transmission electron microscope images of immobilized collagen. Fluctuations in the curvature of collagen fibers formed in-vitro (with or without recombinant DDR2) were analyzed to ascertain their PL. The PL values and fiber-diameter measurements were utilized to estimate Young's Modulus (E) of collagen fibers. Our results show that DDR2 significantly reduced PL and E of collagen fibers. We further found that PL for native collagen fibers increases as a function of collagen concentration with little dependence on fiber diameter. These results signify a physiological role of DDR2 in modulating extracellular matrix stiffness, which may be of relevance for tissue engineering and medical implants especially in diseases where DDR2 is upregulated.