Abstract
Survival of cercariae of Transversotrema patialense was enhanced in saline with an ionic concentration equivalent to 25 % sea water whilst infectivity was unchanged up to the maximum salinity tolerance of the fish experimental host ( Brachydanio rerio). Adult survival in vitro increased little at ionic concentrations above 12.5% of that of sea water but fell sharply at lower concentrations. Survival in vitro was not enhanced by sterile conditions and only increased slightly with increasing age of worms. Adult parasites became water sensitive within 5 min of infection. However mechanically decaudated cercariae showed no water sensitivity. To survive on freshwater hosts the adult parasite must experience a degree of physiological isolation from its environment despite its apparent ectoparasitic microhabitat.
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