Abstract
Six Canadian spring wheat genotypes ( Triticum aestivum L.) were used to study the influence of cold and heat treatments on the efficiency of anther culture. Anther callus yield was increased when the anthers were incubated at 32°C for 6 days prior to incubation at 26°C. Cold treatment of whole spikes or separated anthers adversely affected the callusing efficiency of anthers. While adverse effects of short-term cold treatment were reversible by heat treatment, long-term exposure to cold caused irreversible damage. Initial heat treatment allowed the anthers to remain unaffected by subsequent cold treatment. Plant regeneration was obtained from up to 38% of the induced calli and only very few cali produced albinos.
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