Abstract
Recent research shows that bacterial cellulose membranes (BCm), which represent a promising biomaterial for the use in regenerative medicine, can be used as biological support and/or as a system of extended release of bioactive compounds. The objective of this work was to characterize the influence of different carbon sources (CS1 and CS2), obtained from the processing of sugar cane, on the physical and structural characteristics of the BCm. To develop this work, bacterial strains of Gluconacetobacter hansenii (ATCC 23769 ) were cultivated in media containing nitrogen source (5,6g/L) and increasing concentrations of CS1 (2.5, 5 , 7.5, 10, 12.5 and 15%) and CS2 (5, 10, 15, 20, 25 and 30%). The static culture was maintained at room temperature for 14 days. The influence of the carbon sources and the different concentrations was established by analysis of dry matter yield, tumescence capacity and ultra‐structural analysis. The best yield was obtained with the mass concentration of 10% CS1 (0.71 g/L) and 15% CS2 (0.84 g/L) and percentage of tumescence of 70,7% and 71,6 % respectively. The ultra‐structural analysis of the BCm best yield in the two sources showed a difference among the reticular structures. It was concluded that carbon sources obtained from sugar cane provided, in the concentrations mentioned above, favorable conditions for the biosynthesis of BCm by G. hansenii, with potential for the use in regenerative medicine.Grant Funding Source: Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico ‐ CNPq
Published Version
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