Abstract
The effects of a decrease in dietary protein level on the kinetic behaviour of the four NADPH production systems [glucose 6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), malic enzyme (ME), and NADP-isocitrate dehydrogenase (NADP-IDH)] were investigated in four different trout tissues. Typical hyperbolic saturation curves were always obtained for the activity of these enzymes. A low-protein diet caused a significant decrease in the maximum velocity values ( V max) of all the hepatic enzyme systems studied. The decrease in hexose monophosphate dehydrogenases (G6PDH and 6PGDH) was about 35 and 50%, respectively, whereas for ME and NADP-IDH it was about 35 and 25%. No significant changes were found in the Michaelis constant. These kinetic characteristics are compatible with an increase in the catalytic efficiency of these enzymes without there being any changes in their activity ratio values. The kinetic parameters for these enzymes in the kidney, spleen, and gill tissue did not undergo any significant change.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
