Abstract
The effect of D-asparagine administered exogenously on the glial cicatrix formation was studied in experimental spinal stroke model in rats, which was conducted by photothrombosis of vessels in thoracic spinal cord. The experiments were carried out on three groups of animals: group №1 (n = 20) which was a control group; №2 group (n = 20) which included rats with spinal stroke model; group №3 (n = 20) included rats with spinal stroke model that D-Asparagine was administered from 3rd to 6th day of the experiment, with the dosage 21.7 mg/kg for the course of treatment. According to the analysis results of spinal cord micropreparations it is obvious that the density of cell distribution in the spinal cord slices in rats of group №3 (D-asparagine with the dosage 21,7 mg/kg for the course of treatment) is three times lower in comparison with the same index of the rats from group №2 (without D-asparagine), (p≤0,01). Thus, the density of cell distribution in photothrombosis lesions of spinal cord vessels on micropreparations from group №3 (D-asparagine with the dosage 21, 7 mg/kg for the course of treatment) is close to the value from group №1 (control), which means that statistically significant differences between the amount of cells in group №1 (control) and group №3 (administered by D-asparagine) have not been identified (p≥0, 01).
Highlights
Acute spinal cord injury is one of the most actual problems of modern medicine, which is caused by serious neurological disorders, great number of complications accompanied by the damage of spinal cord and extremely low rehabilitation capacity, which leads to the high level of disability in these patients [3]
Groups characteristics: group No1 included 20 rats, whose spinal cord was used as a control sample; group No2 included 20 rats which were exposed to photothrombosis of vessels of thoracic spinal cord; group No3: included 20 rats which were exposed to spinal stroke and were treated by D-Asparagine from the 3rd to the 6th day of the experiment
While examining the micropreparations of spinal cord taken from rats in group No1, longitudinal fascicles of conducting tracts can clearly be seen in the area of incision. (Figure 1, 2)
Summary
Acute spinal cord injury is one of the most actual problems of modern medicine, which is caused by serious neurological disorders, great number of complications accompanied by the damage of spinal cord and extremely low rehabilitation capacity, which leads to the high level of disability in these patients [3]. A key reason for poor prognosis (in terms of the recovery of neurological deficit) in patients with acute spinal cord injury is the formation of glial cicatrix in the area of spinal cord lesion [1, 2]. Glial cicatrix is both a mechanical and molecular (the synthesis of substances which inhibit the growth of axons) barrier for sprouting of axons distal to the lesion [1, 2]. At the same time it is known that the deficiency of Dasparagine racemase causes inhibition of proliferation of immature cells [6, 10]
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