Abstract
Effects of complexing agents on the sensitivity of ethanol production using Saccharomyces cerevisiae were investigated using trans-1,2-diamino-cyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and diethylene triamine pentaacetic acid (DPTA). Addition of 600 ppm CDTA during inoculation produces a maximum stimulation; ethanol production at this concentration was 1.5% (v/v) more than the control cultures (approximately 6%). 1000 ppm CDTA produces maximum effect during propagation which was 2.3% more than the control cultures. When DPTA was introduced during inoculation, 800 ppm DPTA produces a maximum effect, which was 2.9% more than the control cultures. 200 ppm DPTA produces a maximum stimulatory effect of 1.0% more than the control cultures. No significant effect was observed when DPTA was added during fermentation but 700 ppm CDTA increased ethanol production by 1.3% more than the control cultures.
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