The Influence of Caffeine and the −163 A>C CYP1A2 Polymorphism on Peak and Mean Power during a Wingate Test

Abstract

The influence of the cytochrome P450 enzymes, specifically a polymorphism of the CYP1A2 genotype, following caffeine ingestion has been shown to influence aerobic endurance; however, the effect on short‐term anaerobic performance is inconclusive. Individuals with the AA variant are responders to caffeine and those with the AC/CC variant are non‐responders.PURPOSETo examine the effects of caffeine and specific CYP1A2 genotype on anaerobic performance.METHODS10 subjects completed two 30 second Wingate Anaerobic Tests (WAnT30) (resistance = 0.075 kg•BW−1) on the Velotron that were separated by 2 to 7 days. Relative peak power (PP) and relative mean power (MP) were computed by the Velotron software. An oral bolus of caffeine (CAF), 5mg•kg−1, or placebo (PLA), maltodextrin, was given in a randomized and counterbalanced design 60 min prior to testing. Buccal epithelial cells were collected via a mouth rinse of 0.9% NaCl. Genomic extraction was obtained using QiAmp Mini spin columns and cell lysing with proteinase k, followed by PCR amplification with Fast Taq. The restriction enzyme (ApaI) was used to cut fragments. Cut and uncut samples underwent electrophoresis in 1% agarose gel and ultraviolet light photography identified genotype. The data was analyzed using a 2 (condition) × 2 (CYP) ANOVA with repeated measures (p>0.05).RESULTS5 people were AA and 5 people were AC/CC. The results revealed that CAF elicited no ergogenic effects. The main effect of condition, PLA versus CAF, showed no significant difference for PP or MP (p = 0.49). The main effect of CYP1A2, AA or AC/CC, did not reveal power differences for PP or MP (p = 0.96). Follow‐up pairwise comparisons between PLA to CAF for PP (W•kg−1) showed non‐significant D's of 1.17% in AA (10.3 and 10.42) and −0.38% for AC/CC (10.36 and 10.46, respectively). Likewise, MP resulted in non‐significant D's of 3.66% for AA (8.2 and 8.5 W•kg−1, respectively) and 2.24% for AC/CC (8.3 and 8.5, W•kg−1 respectively).CONCLUSIONCaffeine did not produce an ergogenic effect for anaerobic exercise, regardless of an individual's CYP1A2 variant. However, the larger percent increases, specifically in MP, suggest that further research should be conducted, such as increasing sample size and identifying confounding variables such as other receptor sites that may interact with caffeine.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.