Abstract
Surface pH of rat proximal jejunum as measured in vitro by pH-electrode was found to be significantly more acid than bulk bathing media of neutral pH. Below a bulk pH value between pH 4 and 5, surface pH was isohydric with bulk pH but with increasing alkalinity of the bulk pH, surface pH remained consistently more acidic. The maintenance of a low surface pH was sensitive to external medium glucose concentration, particularly between 2 and 10 mM concentration. A Lineweaver-Burk plot of the glucose-dependent decrement in surface pH demonstrated an apparentK m of 2 mM. Anoxia caused a significant elevation in the measured surface pH at 10 mM glucose concentration. Surface pH was found to be sensitive to buffer sodium ion concentration in the jejunum, an effect which was less marked in the distal ileum.
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