The influence of arsenic trioxide combined with cisplatin on the growth and expression of X-linked inhibitor of apoptosis protein, XIAP of human non-small cell lung cancer cells

Abstract

Lung cancer is the leading cancer of malignant tumors in China. It is the direction that people make effect to seek effective therapy of lung cancer. The aim of this study is to investigate the influence of arsenic trioxide (As₂O₃) injection combined with cisplatin (DDP) on the growth, apoptosis and XIAP mRNA of human non-small cell lung cancer cell lines. The influence of growth of human non-small cell lung cancer cell lines H460 induced by As₂O₃ combined with DDP or without DDP was analysed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The apoptosis rate of H460 was measured by flow cytometry. XIAP mRNA level of the cells was detected by RT-PCR before and after treatment of As₂O₃ combined with DDP or without DDP. Compared to individual drug, As₂O₃ injection combined with DDP may obviously inhibit the proliferation of H460 cells, increase the apotosis rate of cells and strengthen inhibition of XIAP mRNA expression in cells. As₂O₃ injection combined with DDP can significantly increase the chemosensitivity on human non-small cell lung cancer, and the mechanism may be related to its inhibition on the expression of XIAP.