Abstract
Incubation of primary neuronal cultures prepared from the hypothalamus and brainstem of neonatal rats with angiotensin II (Ang-II) resulted in a concentration-dependent effect on the incorporation of [ 3H]-tyrosine ([ 3H]-Tyr) into [ 3H]-catecholamines ([ 3H-CA). At concentrations of 1 nM–1μM, Ang-II (60 min. incubation) caused significant decreases (31–52%) in neuronal [ 3H]-CA content compared with controls. Conversely, higher concentrations of Ang-II (10–100 μM; 60 min.) caused significant increases (20–60%) in neuronal [ 3H]-CA content compared with controls. Both of these effects were blocked by co-incubation with the Ang-II receptor antagonist Sar 1IIe 8-Ang-II. These observations demonstrate that neuronal cells in primary culture have the ability to synthesize [ 3H]-CA from [ 3H]-Tyr, and that Ang-II has a receptor-mediated biphasic influence on newly synthesized [ 3H]-CA (norepinephrine and dopamine).
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