The inflammatory injury role of early growth response factor in lung ischemia-reperfusion injury in rat lung transplant model

Abstract

Objective To investigate the inflammatory injury role of early growth response factor (EGR-1) in lung ischemia-reperfusion injury in rat lung transplant model.Methods Rat left lung transplant models were used in our study,and divided into following groups:1 h after reperfusion,2 h after reperfusion and 4 h after reperfusion.The nomal lung tissure served as a control group.Quantitative real-time RT-PCR was used to detect the expression of EGR-1 and IL-1β mRNA in lung tissures.Myeloperoxidase (MPO) activity,bronchoalveolar lavage (BAL) neutrophil count and the wetto-dry (W/D) lung weight ratio were recorded in every group.Results The expression of EGR-1 and IL-1β13 mRNA was obviously increased in groups of 1 h,2 h and 4 h after reperfusion,and significantly higher than in nonlal control group(P＜0.05).The values of MPO activity,BAL neutrophil count and the W/D lung weight ratio were increased obviously in groups of 1 h,2 h and 4 h after reperfusion as compared with those in the normal control group and there was significant difference between these 4 groups (P＜0.05).The expression of EGR-1 mRNA was significantly correlated with the IL-1β mRNA expression in every group (r=0.921,P＜0.01).The EGR-1 mRNA expression had a strong correlation with BAL neutrophil count (r=0.876,P＜0.01).Conclusion EGR-1 is induced by lung fissure ischemia,and may play an important role in the inflammatory injury mediated by IL-1β in lung ischemia-reperfusion injury in the rat lung transplant model. Key words: Lung; Reperfusion injury; Early growth response factor-1; Inflammation