Abstract
The induction of Metallothionein by Cadmium in sea anemone (Aiptasia pulchella) Metallothioneins(MT) are cysteine rich, heat stable and low molecular weight proteins. They have been widely used as biomarkers to reflect the existence of heavy metal pollution. MT also has the potential being used in well-designed environmental monitoring programmes. Sea anemones(Aiptasia pulchella) should reflect the real condition of its surroundings due to its sedentary. In lab, they are normally reproduced by pedal laceration as asexual reproduction. After exposure to different concentrations of cadmium(0, 0.5, 1.0 and 2.0mg/L) for 24, 48 and 96 hours, obvious morphological alterations in Aiptasia pulchella were recorded. Their tentacles turned dark and their body shrinked. By using atomic absorption to measure the Cd concentration, those Cd-exposed sea anemones showed a time-dependent and a dose-dependent cadmium accumulation. From the results of immunoblotting, the amount of metallothioneins in those cd-exposed A. pulchella significantly increased followed by a time-course. The molecular weight of the induced MT is between 11 and 17.
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