Abstract
The induction of lipase activity in the storage tissues of the germinating wheat grain is dependent upon factors emanating from the embryo. In endosperm halves, lipase activity can be induced in the starchy endosperm by one of several nitrogenous compounds, in particular glutamine. Lipase activity in the bran of endosperm halves is induced by indole acetic acid in the presence of the nitrogen source. Both of these induction processes are inhibited by inhibitors of energy metabolism, RNA synthesis and protein synthesis, and by azaserine, a glutamine analogue. The action of indole acetic acid and the nitrogenous compound in inducing lipase activity in isolated bran appears to require the prior action of a cytokinin and some other unidentified factor.
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