Abstract
Catharanthus roseus is containing anticancer alkaloids vincristine and vinblastine as is an important medicinal plant. Several studies have conducted on in-vitro culture of this plant. To optimize the somatic embryogenesis, a factorial based on CRD experiment with 10 replications was conducted. Root, hypocotyl and leaf explants grown in-vitro were transferred and cultured on MS media containing different combinations of 2,4-D, NAA and 2,4-D×BAP. The results revealed that in callogenesis, the interaction effects of root and hypocotyl explants×2,4-D and NAA as well as hypocotyl×(1 mg l-1 NAA+1 mg l-1 BAP) was superior than other treatments (p≤0.01). For calli fresh weight, hypocotyl×NAA and hypocotyl×(1 mg l-1 NAA+1 mg l-1 BAP) was the treatment of choice (p≤0.01). The calli produced were sub-cultured to attain the pre-embryos and somatic embryos. For the number of pre-embryos and somatic embryos; the interaction of hypocotyl×2,4-D was the best and most efficient treatment. Seemingly, the production of somatic embryos is accessible in this plant by the logical management of growth regulator combinations. Furthermore, the production and genetic engineering of the somatic embryos could be a promising trend in the subsequent production of high-valued metabolites from this plant.
Highlights
IntroductionThe most important alkaloids extracted from the shoots of this plant are vincristine and vinblastine, with a well defined anticancer properties (Mujib et al, 2012)
To optimize the somatic embryogenesis, a factorial based on completely randomized design (CRD) experiment with 10 replications was conducted
The results showed that the effect of plant growth regulators and explants, and their interactions were significant (p≤0.01) on the number of preembryos and somatic embryos produced
Summary
The most important alkaloids extracted from the shoots of this plant are vincristine and vinblastine, with a well defined anticancer properties (Mujib et al, 2012). The amount of these compounds in this plant is about 0.0005% of dry weight of the plant and their extraction is costly and time consuming (BarralesCureño et al, 2017). These problems have led the scientists to a new approaches of tissue culture studies in C. roseus L. The first studies on somatic embryogenesis in the Catharanthus roseus (L.) G. The phe nomenon of genotypedependent plant regeneration exists in other plant species (Firoozabady and De Boer, 1993)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
