The indirect estimation of the digestibility of pasture herbage I. Nitrogen and chromogen as faecal index substances

Abstract

1. In seventeen digestibility trials carried out between May and November, 1957, groups of three steers were fed on fresh herbage, cut mainly from mixed swards. Herbage digestibility and the concentrations in faeces of the index substances nitrogen and chromogen were measured over periods of 5 days. Organic matter digestibility ranged from 61·2 to 78·9%.2. The relationship between herbage digestibility and faecal nitrogen concentration could not be described satisfactorily by a single regression equation for all trials, and separate equations were calculated for (a) first-growth herbage and (b) aftermath (second and third growths). For a given faecal nitrogen concentration herbage digestibility was about five units higher for first-growth than for aftermath herbage.3. For the faecalchromogen-digestibilityrelationship the difference between growths was even greater and, again, two equations were required. In most trials the quantity of chromogen excreted was apparently greater than the quantity consumed.4. The ‘seasonal’ differences in faecal index relationships were caused by the fact that later growths of herbage contained considerably greater concentrations of nitrogen and chromogen than did firstgrowth herbage of the same digestibility. There was also some evidence that the relationships may be affected by the application of nitrogenous fertilizers to the sward.5. In order to avoid biases in the indirect estimation of herbage digestibility it appears important to use different regressions for different growths. Although such regressions would be restricted in their application they are likely to be more precise than general equations. In these trials both nitrogen and chromogen regressions had low residual standard deviations of ± 1·5 units of digestibility.