The in-depth revelation of the mechanism by which a downflow Leersia hexandra Swartz constructed wetland-microbial fuel cell synchronously removes Cr(VI) and p-chlorophenol and generates electricity

Abstract

The composite pollution by Cr(VI) and p-chlorophenol (4-CP) has high toxicity and harms water safety. However, research on the effective removal of Cr(VI) and 4-CP composite-polluted wastewater (C&P) and efficient synchronous electricity generation with reclaimed resources is limited. In this study, a downflow Leersia hexandra constructed wetland-microbial fuel cell (DLCW-MFC) was builded to treat C&P, as well as wastewater singularly polluted by Cr(VI) (SC) and 4-CP (SP), respectively, to reveal the mechanism by which DLCW-MFC treats C&P and synchronously generates electricity. The results demonstrate that the cathode layer had a stronger removal effect on pollutants than the middle layer and anode zone layer. Moreover, SC and SP had stronger pollutant removal effects than C&P. Cr(VI) had more competitive with electrons than 4-CP, and they had a synergistic effect on efficient electricity generation. The L.hexandra in SC and SP had a better growth state and lower Cr enrichment concentration than that in C&P. Cr existed in the DLCW-MFC mainly in the form of Cr(III). Gas chromatography-mass spectrometry was used to investigate the degradation pathway of 4-CP in C&P, and indicated that Phenol, 2,4-bis(1,1-dimethylethyl)- and benzoic acid compounds were the main intermediates formed at the cathode, and further mineralized to form medium-long-chain organic compounds to form CO2. The microbial community distribution results revealed that Simplicispira, Cloacibacterium, and Rhizobium are associated with Cr(VI) removal and 4-CP degradation, and were found to be rich in the cathode of C&P. The anode of C&P was found to have more Acinetobacter (1.34%) and Spirochaeta (4.83%) than SC and SP, and the total relative abundance of electricigens at the anode of C&P (7.46%) was higher than that at the anodes of SC and SP. This study can provide a theoretical foundation for the DLCW-MFC to treat heavy metal and chlorophenol composite-polluted wastewater and synchronously generate electricity.