The Increase of Intestinal Glucose Absorption by the Administration of 5% Glucose Solutions at Temperature of 15°C, 25°C, and 40°C in Mice (Mus Musculus)

Abstract

This research aims to prove the difference in the intestinal glucose absorption of mice after administration of 5% glucose solution with a temperature of 15°C, 25°C and 40°C. The design used in this experimental research was Separated Pre-Post Test. The study were conducted on 28 mice (Mus musculus) and were divided into four groups; i.e. control group without glucose administration, the group treated with fluid administration of glucose 5% at temperature of 15°C, 25°C and 40°C per oral. Absorption of glucose was determined by measuring blood glucose levels in Portal Vein after 30 minutes using a glucose meter.The measurement results showed that the mean glucose level in the control group was 149.17 ± 17.62 mg/dl, the group treatment at temperature of 15°C was 207.67 ± 36.61 mg/dl, at temperature of 25°C was 254.33 ±35.52 mg/dl and the temperature of 40°C was 256.5± 34.83 mg/dl. As indicated by the statistical test, there were significant differences between the control group and the group treated with 5% glucose administration at the temperature of 15°C; control group and 25°C; and control group and 40°C. The treatment group at temperature 15°C to 25°C shows significant differences, while at the temperature of 25°C to 40°C the absorption of glucose was increased although it was not statistically significant.In conclusion the increase of temperature causes tendency of increased intestinal glucose absorption, as indicated by elevated levels of glucose and the highest glucose absorption of portal vein occurred at temperature of 40°C of 5% glucose solution, compared to the groups at 15°C and 25°C. This research aims to prove the difference in the intestinal glucose absorption of mice after administration of 5% glucose solution with a temperature of 15°C, 25°C and 40°C. The design used in this experimental research was Separated Pre-Post Test. The study were conducted on 28 mice (Mus musculus) and were divided into four groups; i.e. control group without glucose administration, the group treated with fluid administration of glucose 5% at temperature of 15°C, 25°C and 40°C per oral. Absorption of glucose was determined by measuring blood glucose levels in Portal Vein after 30 minutes using a glucose meter. The measurement results showed that the mean glucose level in the control group was 149.17 ± 17.62 mg/dl, the group treatment at temperature of 15°C was 207.67 ± 36.61 mg/dl, at temperature of 25°C was 254.33 ±35.52 mg/dl and the temperature of 40°C was 256.5± 34.83 mg/dl. As indicated by the statistical test, there were significant differences between the control group and the group treated with 5% glucose administration at the temperature of 15°C; control group and 25°C; and control group and 40°C. The treatment group at temperature 15°C to 25°C shows significant differences, while at the temperature of 25°C to 40°C the absorption of glucose was increased although it was not statistically significant. In conclusion the increase of temperature causes tendency of increased intestinal glucose absorption, as indicated by elevated levels of glucose and the highest glucose absorption of portal vein occurred at temperature of 40°C of 5% glucose solution, compared to the groups at 15°C and 25°C.