The increase of interfollicular epidermal stem cells and regulation of aryl hydrocarbon receptor and its repressors in the skin through hydroporation with anti-aging cocktail.

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Hydroporation is a procedure that involves a subsonic flow of air and microdroplets into the skin. We previously reported that hydroporation treatment with a cocktail solution containing copper-glycyl-L-histidyl-L-lysyl, oligo hyaluronic acid, rhodiola extract, tranexamic acid, and β-glucan yielded positive effects on skin aging. The aim of this study was to evaluate the effects of hydroporation with anti-aging cocktail on interfollicular epidermal stem cells (IFESCs) and expression of aryl hydrocarbon receptor (AhR)/AhR repressor (AhRR) in the skin. Skin samples from six volunteers who were treated with hydroporation were analyzed via confocal microscopic examination. Markers for dermal matrix (procollagen type I and fibrillin-1) and basement membrane (type IV collagen and integrin α6) were increased after treatment. Moreover, there was a significant increase in the expression level of histone deacetylase 1-positive/p63-negative basal cells, which we previously reported as interfollicular epidermal stem cells. The expression level of AhR was significantly decreased, whereas that of AhRR was increased. This indicates an alteration in the interaction between the skin and environment posttreatment. Anti-aging hydroporation treatment recovered the stem cell potential of basal cells. Moreover, this treatment decreased AhR and increased AhRR in the skin, which may protect the skin from the harmful environment.