The Increase of Incomplete Degradation Products of Galactomannan Production by Synergetic Hydrolysis of β-Mannanase and α-Galactosidase

Abstract

An integrated process to increase the yield of incomplete degradation products of galactomannan (GalM) especially for galactomanno-oligosaccharides (GalMOS) was suggested. Trichoderma reesei employed Avicel or GalMOS as a carbon source to produce β-mannanase or α-galactosidase independently, with a result of 3.78 ± 0.12U/mL of β-mannanase activity and 2.45 ± 0.06U/mL of α-galactosidase activity which were obtained, respectively. GalM in Sesbania seed was hydrolyzed simultaneously by a mixture of crude enzyme with β-mannanase and α-galactosidase at a dosage of 20U/g GalM and 15U/g GalM, respectively; the yields of incomplete degradation products of GalM (IDP-GalM) and GalMOS were 78.84% ± 3.14% and 30.94% ± 0.38%, respectively, which was beneficial to improve the biological activity of the incomplete degradation products. The role of α-galactosidase addition in mixture enzymes is to remove the galactose substituents from mannan backbone of GalM and alleviate the steric hindrance of β-mannanase hydrolysis.