Abstract
Typhus rickettsiae incubated with [ 35S]methionine under suitable conditions show a small but highly significant uptake of methionine. Heat-inactivated rickettsiae and particles from normal yolk sac show no uptake under the same conditions. The substances required for optimum uptake are: ATP or ADP, the other nucleoside-5′-monophosphates, DPN, glutamine, the naturally occurring amino acids, and either a protein fraction from yolk sac or a suitable preparation of bovine serum albumin. The maximum uptake observed in different experiments amounts to 7 to 20 mμg methionine per 100 μg rickettsial protein in 24 h.
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