Abstract
1. 1. The effects of 5-fluorouracil, not reversible in the presence of uracil, on Staphylococcus aureus (strain Duncan) were studied. 2. 2. The logarithmic growth of S. aureus (strain Duncan) in the presence of 5-fluorouracil (100 μg/ml) and uracil (200 μg/ml) was retarded by 90% after 60-min incubation. 3. 3. Up to 4% of the UMP in the ribosomal RNA was replaced during 60-min growth, by 5-fluorouracil ribotide: 14% of UMP in the soluble RNA was replaced during this period. 4. 4. The proportions of cell wall, hexose and polysaccharide were unaffected, but the lipid content fell slightly. 5. 5. Total nucleic acid fell by 45% but the total nitrogen (less nucleic acids) remained constant. 6. 6. Continued incorporation of the analogue into soluble RNA was in contrast to its uptake into particulate RNA. 7. 7. Acid-soluble protein from the cell sap showed marked changes in its electrophoretic characteristics. 8. 8. It was concluded that protein synthesis was unabated, with the production of abnormal proteins caused by unnatural nucleic acids; and that ribosome synthesis was halted, although pre-existing ribosomes remained active.
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