The In Vivo Quantitative Assessment of the Effectiveness of Low-Dose Photodynamic Therapy on Wound Healing Using Optical Coherence Tomography.

Hala Zuhayri,Ekaterina A Sandykova,Natalya A Krivova,Tatiana B Lepekhina,Yury V Kistenev,Viktor V Nikolaev

doi:10.3390/pharmaceutics14020399

Hala Zuhayri, Ekaterina A Sandykova + Show 4 more

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https://doi.org/10.3390/pharmaceutics14020399

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Journal: Pharmaceutics	Publication Date: Feb 11, 2022
Citations: 4	License type: CC BY 4.0

Affiliation: National Research Tomsk State University

Abstract

The effect of low-dose photodynamic therapy on in vivo wound healing was investigated using optical coherence tomography. This work aims to develop an approach to quantitative assessment of the wound’s state during wound healing including the effect of low-dose photodynamic therapy using topical application of two different photosensitizers, 5-aminolevulinic acid and methylene blue, and two laser doses of 1 J/cm2 and 4 J/cm2. It was concluded that the laser dose of 4 J/cm2 was better compared to 1 J/cm2 and allowed the wound healing process to accelerate.

Highlights

According to the World Health Organization (WHO), burn wounds result in approximately 180,000 deaths every year and nearly 11 million injuries that require medical treatment worldwide [1]
The p-values were calculated for all groups on all days
4 J/cm2, Low-dose photodynamic therapy (LDPDT) 5-aminolevulinic acid (5-ALA) 1 J/cm2, LDPDT-methylene blue (MB) 4 J/cm2, and LDPDT-MB 1 J/cm2, it was concluded that the laser dose of 4 J/cm2 for LDPDT 5-ALA was definitely better compared to 1 J/cm2 and probably better for LDPDT MB

Summary

Introduction

According to the World Health Organization (WHO), burn wounds result in approximately 180,000 deaths every year and nearly 11 million injuries that require medical treatment worldwide [1]. Wound healing is a complex physiological process at the cellular and molecular levels including the extracellular matrix synthesis, the replacement of type III collagen with type I collagen, and scar tissue formation [3,4,5,6]. These processes are divided into four overlapping stages: coagulation (hemostasis), inflammation, proliferation, and remodeling [7,8]. Some underlying diseases affect the wound healing process including peripheral arterial and venous disease or diabetes; acute wounds may have impaired healing, which can lead to a chronic stage [9,10,11]. 1–6% of the population suffers from chronic wounds [12,13,14]

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