Abstract
The capacity of antibody response in man was evaluated by a single intradermal injection of polymerized flagellin (POL) from Salmonella adelaide. The antibody was titrated in sera (with or without treatment with 2-mercaptoethanol) by microhemaglutination using antigen-sensitized group O red blood cells (RBC). The naturally occurring antibody to POL was detected in 64% of 190 nonimmunized healthy subjects. Its frequency and titer gradually increased with age. In 10 healthy adult subjects and 15 patients with miscellaneous diseases, a good response with both IgM and IgG antibody was induced. The IgM to IgG switch-over was more clearly elucidated in subjects without the natural antibody. In 12 immunodeficient patients, the subjects with T- and/or B-cell dysfunction did not produce or poorly produced the antibody. Four subjects with Wiscott-Aldrich syndrome (WAS) revealed a comparatively high antibody response; two of them became significantly responsive to POL after administration of transfer factor and reimmunization with POL. POL is one of the best applicable test antigens and appears to act as a T-independent immunogen in man.
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