The in vitro transcription units of bacteriophage φX174 : II. In vitro initiation sites of φX174 transcription

Abstract

Employing the 5′ proximal labeling procedure reported previously, and hybridization to Haemophilus endonuclease bacteriophage φX RF DNA fragments, we have detected three major sites of initiation of in vitro φX transcripts. These three sites have been located on the φX174 genetic map in the following regions: (1) near the 5′ end of gene A, (2) near the 5′ end of gene B, and (3) near the 5′ end of gene D.