Abstract
The in vitro release of newly formed protein from the ribosome to the hot trichloroacetic acid precipitable supernatant fraction requires prelabeled ribosomes, ribosomal-free supernatant fraction, ATP, and probably Mg2+. The optimal ATP concentration is six-fold higher than that required for overall amino acid incorporation. Puromycin and deoxyribonuclease stimulate the release of radioactive protein from ribosomes both in the presence and absence of ATP, while chloramphenicol is neither stimulatory nor inhibitory. At least half the radioactivity released from prelabeled ribosomes is not precipitable by trichloroacetic acid. The amount of this material increases with time. ATP is required to effect the release of this trichloroacetic acid non-precipitable component.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects
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