The in vitro propagations of the raspberry cultivar Citria

Abstract

This paper presents aspects regarding the in vitro propagation of the yellow raspberry cultivar Citria. For culture initiation, two variants of media were used, both having Murashige-Skoog (MS) (1962) as basal medium and, as growth regulators, 1 mg/l 6-benzylaminopurine for variant 1 and 2 mg/l Zeatin for variant 2. In order to establish the optimal medium from the point of view of multiplication rate as well as from an economical point of view, experiments were done with 3 variants of media, variants 1 and 2 had, as basal medium MS salts, Myo inositol-100 mg/l, Vitamin B1- 1 mg/l, Vitamin B6 - 0.5 mg/l, Nicotinic Acid - 0.5 mg/l, FeNaEDTA stock solution- 5ml/l, and variant 3 had, as basal medium, Woody Plant Medium (WPM) salts, Myo-inositol-100 mg/l, Vitamin B1- 2 mg/l, Vitamin B6 - 1 mg/l, Nicotinic Acid - 1 mg/l, Sequestrene 138- 100mg/l. As growth regulators, 0.7 mg/l BAP was used in variant 1, 0.1 mg/l TDZ in variant 2 and 1 mg/l Zeatin in variant 3. All variants had commercial crystal sugar as carbon source and Plant Agar as gelling agent, pH was adjusted to 5.7 for variants 1 and 2 and 5 for variant 3.