The in vitro O2-consumption of kidney from normal and sublimate intoxicated rats.

Abstract

The in vitro O2-consumption of kidney slices from normal and sublimate intoxicated rats has been studied by means of Warburg's method. It has been demonstrated previously that the weight of kidneys from sublimate intoxicated rats varies with a positive linear correlation to urine output. the traditional units of Warburg's method, in which O2-consumption is related to dry weight or any known constituent of the investigated tissue, were not suitable for the present experiment. A new unit is therefore introduced, and is defined as microliter O2-consumption in kidneys per g body weight per hour, here called -qo2This unit is reduced with increasing age of the rat, and varies only slightly within an even age group of normal rats. in sublimate intoxicated rats, -qo2 has a close positive linear correlation to the specific gravity of the urine. in a series of rats given 4 mg sublimate per kg body weight, -qo2 was reduced to nearly one half of normal at the time of greatest polyuria. There was no morphological sign...