The in vitro motility of human gliomas increases with increasing grade of malignancy

Abstract

Malignant gliomas are a highly invasive disease with a dismal prognosis despite aggressive therapeutic interventions. The authors tested the hypothesis that increasing in vitro motility of human glial tumors correlates with increasing grade of malignancy. A radial dish assay was used to quantitatively assess the brain tumor cell motility of 14 low passage cell lines derived from human glial tumors of varied malignancy, and 3 cell lines derived from human glia. The egress of cells from a central region of high tumor cell density to a region of lower tumor cell density was determined at various time points. A motility coefficient (MC), the slope of distance traveled against time, was generated by simple linear regression analysis. The MC increased with the increased histologic grade of malignancy. Generation of a t-statistic was used to determine the significance of differences in motility among 5 histopathologic groups: 10 glioblastoma (World Health Organization Grade IV) cell lines (mean MC, 0.00396), 2 mixed anaplastic oligoastrocytoma (Grade III) cell lines (mean MC, 0.00382), 1 anaplastic astrocytoma (Grade III) cell line (mean MC, 0.00295), 1 Grade I glioma cell line (mean MC, 0.00206), and 3 human glial cell lines (mean MC, 0.00110). The radial dish assay provided a reproducible method for quantitatively assessing brain tumor cell motility. The higher MC observed with the malignant human glioma cell lines correlated with the tendency of these tumors to invade into adjacent brain tissue. The potential to inhibit glial tumor motility may provide an important therapeutic avenue in the future.