Abstract
The thymic anlagen of 10‐day‐old chicken embryos showed a reproducible development in organ culture, assayed as the number of lymphoid cells obtained per anlage. The time dependent increase of the number of lymphoid cells exhibited an exponential phase during culture on days 0–6 followed by a plateau phase during days 8–12. The characteristic growth curve was not significantly changed by subcultivation of whole or divided thymic anlagen or medium changes, in order to improve the culture conditions. The lymphoid development of the thymic anlagen in organ culture required chicken serum as medium supplement. The number of lymphoid cells per anlage showed an approximately linear relation to the logarithm of the serum concentration within a range of 2.5–20 per cent serum. Additional and removal of organ culture medium serum supplement indicated that serum was necessary both for the initiation and maintenance of the thymic lymphoid development.
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Schedule a callMore From: Acta pathologica et microbiologica Scandinavica. Section A, Pathology
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