Abstract
The pentose phosphate pathway is the metabolic pathway where NADPH, the reducing force in metabolism, and ribose 5-phosphate, the building block of DNA and RNA, are produced. In this study, the in vitro effects of 5-fluorouracil and Tamoxifen chemotherapeutic agents on glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD), which are key enzymes of the pentose phosphate pathway, were investigated. In the first stage of the study, G6PD and 6PGD enzymes were purified from rat erythrocytes using 2ʹ, 5ʹ-ADP Sepharose-4B affinity chromatography. The control of enzyme activities was determined spectrophotometrically at 340 nm wavelength according to the Beutler method. Then, the in vitro effects of 5-FU and Tamoxifen agents, which are widely used in chemotherapy, on enzyme activities were investigated. The results of the study showed that 5-FU increased the activity of both G6PD and 6PGD enzymes in the concentration range of 0.19-1.9 mM and Tamoxifen in the concentration range of 26-260 µM.
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