Abstract
The biosynthesis of 11β-hydroxy-androst-4-ene-3,l 7-dione (11βOHA) was studied, using homogenates of normal and hyperplastic human adrenal tissue. Incubations with radioactive cortisol, progesterone, and dehydroepiandrosterone (DHA) were performed in the presence of ATP and NADPH to determine their conversion to 11βOHA. Incubation of the hyper-plastic tissue with 14C-DHA and 3H-cortisol showed the conversion to 11βOHA to be 26.8 and 4.8%, respectively, in 60 minutes. The conversion by the normal tissue was 31 and 7.2%. In experiments with 14C-DHA and 3H-progesterone as substrates, the conversion to 11βOHA from DHA by the hyperplastic tissue was 38, 40, and 47.3% at 10, 30, and 60 minutes. The conversion by the normal tissue was 22% at 30 minutes. At the same time intervals, the conversions of 3H-progesterone to 11βOHA by the hyperplastic tissue were 1.4, 1.8, and 2.8%. The normal tissue converted 2.7% of the 3H-progesterone to 11βOHA in 30 minutes. Final 3H/14C ratios indicated that some of the 11βOHA for...
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