The in silico identification of hemicellulase producing microorganisms found in animal manure

Abstract

The purpose of this study is to find out the high activity of xylanase producing microorganisms in order to reduce the cost of raw materials that used in several commercial industries like paper pulp bleaching ,bioconversion of lignocellulosic biomass into bio fuels etc.This study was conducting to characterize and identify most probably high efficient hemicellulose degrading enzymes from bacterial and fungal species by using bioinformatics methodologies.in this study 27 xylanase producing bacterial sequence and 13 xylanase producing fungal sequence were retrieved from N CBI data bank (http://www.ncbi.org). The predicted microorganisms were further analyze by retrieving protein sequence from uniprot.This study concluded that Geobacillus strearothermophilus and Claviceps purpurea, exhibit a high level of specific enzyme activity as compared to target bacterial and fungal sp. Myceliophthora thermophila and claviceps purpurea respectively at the favorable temperature of 60◦C with optimal neutral pH. The identified enzymes will be helpful in the reduction of cost and energy required in conversion of biomass or in various other industrial sectors for the processing of raw material.