Abstract
Turkey with 18 oak (Quercus) species is one of the richest country according to species number and diversity. The most important reason of the species diversity in Turkey is its location and geomorphological structure which increase climatic effects and seperate Turkey into different phytogeographic regions. Furthermore, hybridization behaviours which frequently observed between oak species, genetic drift, gene flow and ecological factors cause morphological variations in the plants species. All of these factors make it difficult to define the species concept for plant groups like oaks. Therefore, the region covering 18S rRNA gene/ ITS1/ 5.8S rRNA gene/ ITS2/ 25S rRNA gene and secondly intergenic spacer (IGS)/ 5S rRNA gene for barcoding were obtained from genbank and used as a useful tool for the determination and solution of the phylogenetic relations of taxonomically problematic species, also these barcoding regions were compared with each other according to species recognition ability for oak species. As a result, it can be stated that both barcoding regions have high variable sites based on sequence information to identify the species and evaluate relationships of species studied.
 Bangladesh J. Plant Taxon. 27(2): 261-271, 2020 (December)
Highlights
DNA barcoding is very important molecular approach for definition biodiversity, evolutionary studies and especially for identifying species with taxonomically problems
The objective of this study is to evaluate phylogenetic relationships of Quercus species by using the 18S rRNA gene/ ITS1/ 5.8S rRNA gene/ ITS2/ 25S rRNA gene and intergenic spacer (IGS)/ 5S rRNA gene from genbank and compare these barcoding regions according to species recognition ability
Eighteen oak species belonging to three subgeneric sections (Quercus, Cerris and Ilex) currently occur in Turkey that is one of the richest country with species diversity and number (Yaltirik, 1984)
Summary
DNA barcoding is very important molecular approach for definition biodiversity, evolutionary studies and especially for identifying species with taxonomically problems. DNA sequences prefered in DNA barcoding must have sufficient variability in grouping of species according to common characteristics and separation of taxonomically closely related species. Short DNA sequence that contain sufficient sequence variation to distinguish species is used for DNA barcode as molecular marker (Kress and Erickson, 2007). Internal transcribed spacer (ITS) regions of rDNA genes in genomic DNA are the sequences prefered the most commonly for plant molecular systematic studies (Baldwin et al, 1995; Alvarez and Wendel, 2003; Bailey, 2003; Sramko, 2008; Sramko et al, 2014). The external transcribed spacers (ETS) and the intergenic spacer (IGS) are widely utilized in phylogenetics in addition to ITS region
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