The impacts of garlic and voluntary training alone or together on myocardial miR-126 and miR-210 gene expressions and angiogenesis in healthy rats.

Rafighe Ghiasi,Alireza Alihemmati,Roya Naderi

doi:10.34172/jcvtr.2020.33

Rafighe Ghiasi, Alireza Alihemmati + Show 1 more

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https://doi.org/10.34172/jcvtr.2020.33

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Abstract

Introduction: microRNAs (miRs) play a critical role in both physiological and pathological processes. Recent studies have shown that garlic and exercise training have many beneficial effects in different disorders including cardiovascular disease. However, their mechanisms have not been fully understood. This study sought to investigate the impact of garlic and voluntary training alone or together on themiR-126 and miR-210 gene expressions and cardiac angiogenesis. Methods: Male Wistar rats were divided into four groups (n=7): (1) Control, (2) Garlic, (3) Exercise, and (4) Garlic+ Exercise. Animals were gavaged with raw fresh garlic homogenate (250 mg/kg body weight/day) or were subjected to voluntary training alone or together for about 6 weeks. The expressions of miR-126 and miR-210 in the heart tissue were measured by real-time PCR and lipid profile in serum was assessed by enzymatic kits. Angiogenesis was determined by immuno staining detection of PECAM-1 and CD31 in the heart tissue. Results: Garlic and exercise up-regulated myocardial miR-126 (P < 0.01), miR-210 (P < 0.001)expressions, and angiogenesis (P < 0.001) which was evidenced by higher CD31 expression. Besides, combination of garlic and exercise amplified their effects on those parameters (P < 0.001). Moreover, both voluntary exercise and garlic alone (P < 0.01) or together (P < 0.001) markedly modulated serum lipid profile. Conclusion: Voluntary exercise and garlic treatment for 6 weeks enhanced myocardial angiogenesis. These alterations were partly due to the increment of miR-126 and miR-210 expressions in the heart tissue in relation to improvement in lipid profile.

Highlights

MicroRNAs play a critical role in both physiological and pathological processes
Impacts of garlic and voluntary training on miR-126 gene expression in the myocardium As shown in Figure 1, the myocardial miR-126 expression level was significantly increased in Garlic and Exercise groups alone (P < 0.01) and in combination (P < 0.001) in comparison to the Control group
Treatment with Garlic and performing voluntary exercise together demonstrated significantly (P < 0.05) higher level of miR126 gene expression compared with garlic treatment and voluntary exercise alone

Summary

Introduction

MicroRNAs (miRs) play a critical role in both physiological and pathological processes. Conclusion: Voluntary exercise and garlic treatment for 6 weeks enhanced myocardial angiogenesis These alterations were partly due to the increment of miR-126 and miR-210 expressions in the heart tissue in relation to improvement in lipid profile. It was known that miR-126 was considered as a pivotal endothelialspecific miR, involved in heart angiogenesis via vascular endothelial growth factor (VEGF) pathway as an important angiogenic factor.[3] recent studies have focused on the role of miR-210 in angiogenesis process.[5] miR-210, a master hypoxamir, induces an angiogenic response in endothelial cells and enhances microcirculation under both physiological and pathophysiological situations.[5] Exercise training is a good strategy for the cardiovascular system in part through improved lipid profile, increased blood flow in vessels by structural changes in vasoreactivity of the coronary arteries.[3] Even so, Da Silva et al has been demonstrated that swimming training promoted angiogenesis in the heart of rats.[3]

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