THE IMPACT OF TWO ANTICOAGULANTS ON ERYTHROCYTES MORPHOLOGY IN DIFFERENT VERTEBRATE SPECIES

Daniel Cocan,Radu Constantinescu,Vioara Mireşan,Alexandru Giurgiu,Călin Laţiu,Tudor Păpuc,Camelia Răducu,Paul Uiuiu

doi:10.46909//journalalse-2021-023

Abstract

In this study, we aimed to highlight the influence of anticoagulants on erythrocyte morphometry in different vertebrate species. Anticoagulants are a category of substances that inhibit blood clotting through various mechanisms. Due to this property, they are used to collect blood samples for a wide range of laboratory tests. The literature mentions that the use of anticoagulants produces morphological changes of erythrocytes, thus influencing results. Blood samples were collected from three warm-blooded vertebrate species (horse, rabbit, and chicken) and one lower vertebrate species with nucleated erythrocytes (fish) in vacutainers with Heparin and EDTA (ethylenediaminetetraacetic acid), in a normal concentration and a double concentration. At the time of harvesting, control smears were performed. In order to be able to compare the effects produced by anticoagulants on the morphology of erythrocytes, they were evaluated morphometrically at intervals of 3, 6, and 24 hrs. after harvest. The following features were evaluated using the Toup View software: length, width, surface and perimeter of erythrocytes for species with anucleated erythrocytes. The same characteristics were evaluated in the nucleus for species with nucleated erythrocytes. The data obtained were processed with statistical programs to highlight changes in erythrocyte morphology produced by anticoagulants.

Highlights

Blood sampling is useful in the diagnosis of clinical diseases and in the routine management of animal welfare (Harikrishnan et al, 2018)
Blood samples were collected from three warm-blooded vertebrate species and one lower vertebrate species with nucleated erythrocytes in vacutainers with Heparin and EDTA, in a normal concentration and a double concentration
Based on the conducted experiments, it can be seen that the erythrocyte surface in horses has similar values when collected on EDTA in the recommended dose and when analysed after 24 hrs. from sampling

Summary

Introduction

Blood sampling is useful in the diagnosis of clinical diseases and in the routine management of animal welfare (Harikrishnan et al, 2018). Research in the field of haematology and blood biochemistry in animals often involves collecting samples in anticoagulant media to maintain the liquid state of blood for a longer period (Barrelet and Ricketts, 2002). Anticoagulant use, according to some studies, frequently results in erroneous results, in terms of erythrocyte indices, due to the morphological alterations that. INFLUENCE OF ANTICOAGULANTS ON ERYTHROCYTES MORPHOLOGY IN VERTEBRATE SPECIES anticoagulants cause at the cellular level (Walencik and Witeska, 2007). Anticoagulants are substances that inhibit the clotting process of blood or plasma, both in vivo and in vitro. The goal is to keep the sample, as well as the drug or its metabolites of interest, in a stable state from the moment of collection through analysis (Kulkarni et al, 2016)

Objectives

Methods

Results

Conclusion